Hana Fleková initially studied cello at the Prague Conservatory and later at the Academy of Performing Arts (AMU) in Prague. She spent some time in London where she received her education in the performance of viola da gamba.
Currently, she is devoted to the authentic interpretation of 17th and 18th century music, is a member of the leading Czech music ensembles Collegium 1704, Collegium Marianum, For Several Friends and Musica Florea. Fleková collaborates with other groups such as Ensemble Tourbillon and Ensemble Inégal. She records her music with both Czech and foreign record labels.